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Despite these problems, there is still considerable interest in computational pK a determination methods in such fields as drug discovery, where the method might be used to reduce uncertainty of the physical/chemical properties of (modifications of) molecules without even having to synthesize all of them
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The intrinsic pathway can be triggered either as a result of mPTP opening, or activation of the proapoptotic proteins of the B-cell lymphoma 2 (Bcl-2) family, Bax and Bak [192] after the removal of the block of antiapoptotic proteins Bcl-2 and Bcl-XL [193]
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